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<div>Thanks for the reply. It worked. I was finally able to open the Spim acquisition plugin and take images with the microscope. I am running Fiji on a Windows 7 32 bit machine with the Stradus laser. I still have an issue. The Stradus laser power cannot
be modulated by the Spim acquisition window. The laser power slider doesn't do anything. Any advice? </div>
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<div>Cheers</div>
<div>mike</div>
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<span style="font-weight:bold">From: </span>Zuomin Zhao <<a href="mailto:zuomin@ee.oulu.fi">zuomin@ee.oulu.fi</a>><br>
<span style="font-weight:bold">Date: </span>Tuesday, October 14, 2014 2:35 AM<br>
<span style="font-weight:bold">To: </span>Michael Redd <<a href="mailto:mredd@cores.utah.edu">mredd@cores.utah.edu</a>><br>
<span style="font-weight:bold">Subject: </span>RE: stradus laser in OpenSPIM<br>
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<p class="MsoNormal"><span lang="EN-US" style="font-size: 11pt; color: rgb(31, 73, 125); font-family: Calibri, sans-serif; ">Oh, Yes. we solved the problem, as the suggestion
<a href="http://openspim.org/pipermail/openspim/2014-May/000408.html">http://openspim.org/pipermail/openspim/2014-May/000408.html</a><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size: 11pt; color: rgb(31, 73, 125); font-family: Calibri, sans-serif; "><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size: 11pt; color: rgb(31, 73, 125); font-family: Calibri, sans-serif; ">Since the Stradus device adapter is "secret", my ability to help is limited.<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size: 11pt; color: rgb(31, 73, 125); font-family: Calibri, sans-serif; ">My suggestion is:<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size: 11pt; color: rgb(31, 73, 125); font-family: Calibri, sans-serif; ">- First, please make sure you are using the Stradus device adapter<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size: 11pt; color: rgb(31, 73, 125); font-family: Calibri, sans-serif; ">(mmgr_dal_Stradus.dll) from the 5 February 2014 nightly build of<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size: 11pt; color: rgb(31, 73, 125); font-family: Calibri, sans-serif; ">Micro-Manager[1].<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size: 11pt; color: rgb(31, 73, 125); font-family: Calibri, sans-serif; ">- Run OpenSPIM, but before clicking "Acquire SPIM Image", create a pixel<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size: 11pt; color: rgb(31, 73, 125); font-family: Calibri, sans-serif; ">size:<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size: 11pt; color: rgb(31, 73, 125); font-family: Calibri, sans-serif; ">- On Micro-Manager, click "Tools -> Pixel Size Calibration..."<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size: 11pt; color: rgb(31, 73, 125); font-family: Calibri, sans-serif; ">- Click "New"<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size: 11pt; color: rgb(31, 73, 125); font-family: Calibri, sans-serif; ">- Set Label to "Uncalibrated"<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size: 11pt; color: rgb(31, 73, 125); font-family: Calibri, sans-serif; ">- Set Pixel Size (um/pixel) to 1.00<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size: 11pt; color: rgb(31, 73, 125); font-family: Calibri, sans-serif; ">- Across from "Core - Initialize" click "Use" checkbox.<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size: 11pt; color: rgb(31, 73, 125); font-family: Calibri, sans-serif; ">- Click "OK".<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size: 11pt; color: rgb(31, 73, 125); font-family: Calibri, sans-serif; ">- Try "Acquire SPIM Image" again.<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size: 11pt; color: rgb(31, 73, 125); font-family: Calibri, sans-serif; "><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size: 11pt; color: rgb(31, 73, 125); font-family: Calibri, sans-serif; ">-Zuomin<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size: 11pt; color: rgb(31, 73, 125); font-family: Calibri, sans-serif; "><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span lang="EN-US" style="font-size: 10pt; font-family: Tahoma, sans-serif; ">From:</span></b><span lang="EN-US" style="font-size: 10pt; font-family: Tahoma, sans-serif; "> Michael Redd [<a href="mailto:mredd@cores.utah.edu">mailto:mredd@cores.utah.edu</a>]
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<b>Sent:</b> Monday, October 13, 2014 10:54 PM<br>
<b>To:</b> Zuomin Zhao<br>
<b>Cc:</b> <a href="mailto:openspim@openspim.org">openspim@openspim.org</a><br>
<b>Subject:</b> Re: stradus laser in OpenSPIM<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><span style="font-size: 10.5pt; color: black; font-family: Calibri, sans-serif; ">I am experiencing the same problem that you described in a posting on the Openspim forum in June:<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size: 10.5pt; color: black; font-family: Calibri, sans-serif; "><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size: 10.5pt; color: black; font-family: Calibri, sans-serif; "><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size: 10.5pt; color: black; font-family: Calibri, sans-serif; ">"</span><span class="apple-style-span"><span style="font-size: 13.5pt; color: black; font-family: 'Courier New'; ">Dear Sir,</span></span><span style="font-size: 10.5pt; color: black; font-family: Calibri, sans-serif; "><o:p></o:p></span></p>
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<pre style="white-space:pre-wrap"><span style="color:black"><o:p> </o:p></span></pre>
<pre><span style="color:black"> <o:p></o:p></span></pre>
<pre><span style="color:black"><o:p> </o:p></span></pre>
<pre><span style="color:black">We used a stradus laser in OpenSPIM. But we cannot open the Acquire OpenSPIM<o:p></o:p></span></pre>
<pre><span style="color:black">option from Micro-manager. The settings can be seen in attachments. It<o:p></o:p></span></pre>
<pre><span style="color:black">always shows error like "Error in device VLTStradus: Duplicate property<o:p></o:p></span></pre>
<pre><span style="color:black">names are not allowed"<o:p></o:p></span></pre>
<pre><span style="color:black"><o:p> </o:p></span></pre>
<pre><span style="color:black"> <o:p></o:p></span></pre>
<pre><span style="color:black"><o:p> </o:p></span></pre>
<pre><span style="color:black">Now we use one stradus laser with wavelength 561 nm, I cannot understand<o:p></o:p></span></pre>
<pre><span style="color:black">where "duplicate property names" happens. <o:p></o:p></span></pre>
<pre><span style="color:black"><o:p> </o:p></span></pre>
<pre><span style="color:black"> <o:p></o:p></span></pre>
<pre><span style="color:black"><o:p> </o:p></span></pre>
<pre><span style="color:black">Another problem is the information in Corelog20140430 that "Startup script<o:p></o:p></span></pre>
<pre><span style="color:black">file (C:\Users\Admin\Desktop\Fiji.app/MMStartup.bsh) not present." I do not<o:p></o:p></span></pre>
<pre><span style="color:black">know why miss this file MMSartup.bsh. How handle it?<o:p></o:p></span></pre>
<pre><span style="color:black"><o:p> </o:p></span></pre>
<pre><span style="color:black"> <o:p></o:p></span></pre>
<pre><span style="color:black"><o:p> </o:p></span></pre>
<pre><span style="color:black">Could you help us? Thank you very much.<o:p></o:p></span></pre>
<pre><span style="color:black"><o:p> </o:p></span></pre>
<pre><span style="color:black"> <o:p></o:p></span></pre>
<pre><span style="color:black"><o:p> </o:p></span></pre>
<pre><span style="color:black">Best regards,<o:p></o:p></span></pre>
<pre><span style="color:black"><o:p> </o:p></span></pre>
<pre><span style="color:black"> <o:p></o:p></span></pre>
<pre><span style="color:black"><o:p> </o:p></span></pre>
<pre><span style="color:black">Zuomin Zhao<o:p></o:p></span></pre>
<pre style="white-space:pre-wrap"><span style="color:black"><o:p> </o:p></span></pre>
<pre style="white-space:pre-wrap"><span style="color:black"><o:p> </o:p></span></pre>
<pre style="white-space:pre-wrap"><span style="color:black">Were you able to solve this problem and move on?<o:p></o:p></span></pre>
<pre style="white-space:pre-wrap"><span style="color:black"><o:p> </o:p></span></pre>
<pre style="white-space:pre-wrap"><span style="color:black">Cheers<o:p></o:p></span></pre>
<pre style="white-space:pre-wrap"><span style="color:black">mike<o:p></o:p></span></pre>
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<p class="MsoNormal"><span style="font-size: 10.5pt; color: black; font-family: Calibri, sans-serif; "><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size: 11pt; color: black; font-family: Calibri, sans-serif; ">From:
</span></b><span style="font-size: 11pt; color: black; font-family: Calibri, sans-serif; ">Zuomin Zhao <<a href="mailto:zuomin@ee.oulu.fi">zuomin@ee.oulu.fi</a>><br>
<b>Date: </b>Monday, October 13, 2014 12:44 AM<br>
<b>To: </b>Michael Redd <<a href="mailto:mredd@cores.utah.edu">mredd@cores.utah.edu</a>><br>
<b>Subject: </b>RE: stradus laser in OpenSPIM<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size: 10.5pt; color: black; font-family: Calibri, sans-serif; "><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size: 11pt; color: rgb(31, 73, 125); font-family: Calibri, sans-serif; ">Dear Michael,</span><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size: 11pt; color: rgb(31, 73, 125); font-family: Calibri, sans-serif; "> </span><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size: 11pt; color: rgb(31, 73, 125); font-family: Calibri, sans-serif; ">What ”duplicate properties”? Could you tell more detail about your problem?</span><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size: 11pt; color: rgb(31, 73, 125); font-family: Calibri, sans-serif; "> </span><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size: 11pt; color: rgb(31, 73, 125); font-family: Calibri, sans-serif; ">Regards,</span><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size: 11pt; color: rgb(31, 73, 125); font-family: Calibri, sans-serif; "><br>
Zuomin</span><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size: 11pt; color: rgb(31, 73, 125); font-family: Calibri, sans-serif; "> </span><span style="color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><b><span lang="EN-US" style="font-size: 10pt; color: black; font-family: Tahoma, sans-serif; ">From:</span></b><span lang="EN-US" style="font-size: 10pt; color: black; font-family: Tahoma, sans-serif; "> Michael Redd [<a href="mailto:mredd@cores.utah.edu">mailto:mredd@cores.utah.edu</a>]
<br>
<b>Sent:</b> Saturday, October 11, 2014 3:09 AM<br>
<b>To:</b> <a href="mailto:zuomin@ee.oulu.fi">zuomin@ee.oulu.fi</a><br>
<b>Cc:</b> <a href="mailto:openspim@openspim.org">openspim@openspim.org</a><br>
<b>Subject:</b> stradus laser in OpenSPIM</span><span style="color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="color:black"> <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size: 10.5pt; color: black; font-family: Calibri, sans-serif; ">Dear Dr. Zhoa,</span><span style="color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size: 10.5pt; color: black; font-family: Calibri, sans-serif; ">I am building a SPIM microscope with a stradus laser. I am experiencing the same "duplicate properties" error that you reported last June on the openspim
forum. Did you solve this problem? If so I would be much obliged if you would share the solution with me.</span><span style="color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size: 10.5pt; color: black; font-family: Calibri, sans-serif; "> </span><span style="color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size: 10.5pt; color: black; font-family: Calibri, sans-serif; ">thanks-- </span><span style="color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size: 10.5pt; color: black; font-family: Calibri, sans-serif; ">Michael Redd</span><span style="color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size: 10.5pt; color: black; font-family: Calibri, sans-serif; ">Imaging Specialist</span><span style="color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size: 10.5pt; color: black; font-family: Calibri, sans-serif; ">Microscopy Core Facility</span><span style="color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size: 10.5pt; color: black; font-family: Calibri, sans-serif; ">University of Utah</span><span style="color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size: 10.5pt; color: black; background-image: initial; background-attachment: initial; background-origin: initial; background-clip: initial; background-color: white; font-family: Calibri, sans-serif; background-position: initial initial; background-repeat: initial initial; ">Radiobiology
Bldg, Room 55</span><span style="font-size: 10.5pt; color: black; font-family: Calibri, sans-serif; "><br>
<span style="background:white">Salt Lake City, Utah</span></span><span style="color:black"><o:p></o:p></span></p>
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