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<div class="moz-cite-prefix">Hi Veli-Pekka,<br>
<br>
On 8/14/2014 7:01 AM, Veli-Pekka Ronkainen wrote:<br>
<blockquote
cite="mid:dd7029c83d4a472a841d50a2b2cb83a6@nippu12.univ.yo.oulu.fi"
type="cite">However, now after using the Fiji updater Picard
USB-stage rotation angle coordinates are not right anymore.
Changing angle from -180 deg to 180 deg rotates the sample
holder only ~180 degrees, and rotation in general spins the
sample much less than indicated by the slider. In
addition, we can not access and modify old .cfg-files, so that
it would be possible to change step size of the twister from 1.8
to 1, as it was discussed earlier: <a moz-do-not-send="true"
href="http://openspim.org/pipermail/openspim/2014-July/000482.html">
http://openspim.org/pipermail/openspim/2014-July/000482.html</a> .</blockquote>
As you suspected, this is because of the change in step size.<br>
<br>
<blockquote
cite="mid:dd7029c83d4a472a841d50a2b2cb83a6@nippu12.univ.yo.oulu.fi"
type="cite">Modification of an existing configuration starts
with an error "Error in device Picard Z Stage: Unable to
communicate with the device" followed by lots of COM and "No
device with label _device name_" -errors. Creating a new
configuration doesn't help since it is not possible to
add Picard stage z-axis to the config file or modify the serial
number.
<div
style="font-size:12pt;color:#000000;background-color:#FFFFFF;font-family:Calibri,Arial,Helvetica,sans-serif;">
<div style="font-size:12pt; color:#000000;
background-color:#FFFFFF;
font-family:Calibri,Arial,Helvetica,sans-serif">
</div>
</div>
</blockquote>
The error when modifying a configuration or creating new
configurations is a different problem entirely, and one I wasn't
aware of. If you could, please, attach a CoreLog or two from after
the update, when you've received these errors. (If you can't tell
which one contains these errors, you can open them and search, or
just run the program again and produce a new one.) This would go a
long way towards figuring out what's causing this problem.<br>
<br>
But in the meantime, an easy, dirty solution to the problem
interfering with your experiments is to open a working
configuration file in Notepad. You should see a line in there that
reads something like<br>
<br>
"Device,Picard Twister,StepSize,1.8"<br>
<br>
Changing the 1.8 to 1 should resolve at least the rotational stage
problem.<br>
<br>
<blockquote
cite="mid:dd7029c83d4a472a841d50a2b2cb83a6@nippu12.univ.yo.oulu.fi"
type="cite">
<div
style="font-size:12pt;color:#000000;background-color:#FFFFFF;font-family:Calibri,Arial,Helvetica,sans-serif;">
<div style="font-size:12pt; color:#000000;
background-color:#FFFFFF;
font-family:Calibri,Arial,Helvetica,sans-serif">
<p>We've tried to copy the latest Picard .dll-files from the
recent Micro-Manager nightly build but the problem
persists. Are there some other files we should copy and
replace as well? Of course we can still download and
configure the non-updated package from openspim.org and be
happy using that older version without any problems. Still
it would be nice to be up-to-date. Anyone having the same
problems or some solutions? Thanks!</p>
</div>
</div>
</blockquote>
You shouldn't need any other files; hopefully the above is all it
takes to get things running until I can figure out what's causing
the trouble with editing configurations.<br>
<br>
Hope this helps,<br>
Luke Stuyvenberg<br>
<br>
P.S. If you get a few moments, please feel free to update
<a class="moz-txt-link-freetext" href="http://openspim.org/Who_has_an_OpenSPIM%3F">http://openspim.org/Who_has_an_OpenSPIM%3F</a> with your location and
information! We love hearing about people using the system.<br>
<br>
On 8/14/2014 7:01 AM, Veli-Pekka Ronkainen wrote:<br>
</div>
<blockquote
cite="mid:dd7029c83d4a472a841d50a2b2cb83a6@nippu12.univ.yo.oulu.fi"
type="cite">
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<div style="font-size:12pt; color:#000000;
background-color:#FFFFFF;
font-family:Calibri,Arial,Helvetica,sans-serif">
<p>Dear all,</p>
<p><br>
</p>
<p>We've been scanning some mouse tissue test samples with our
dual-side illumination OpenSPIM for a short while now, and
we are very happy with the first results!
<br>
</p>
<p><br>
</p>
<p>We have used the original software package downloaded
from openspim.org without any updates, and it has been
working fine. However, now after using the Fiji updater
Picard USB-stage rotation angle coordinates are not right
anymore. Changing angle from -180 deg to 180 deg rotates the
sample holder only ~180 degrees, and rotation
in general spins the sample much less than indicated by the
slider. In addition, we can not access and modify old
.cfg-files, so that it would be possible to change step size
of the twister from 1.8 to 1, as it was discussed earlier: <a
moz-do-not-send="true"
href="http://openspim.org/pipermail/openspim/2014-July/000482.html">
http://openspim.org/pipermail/openspim/2014-July/000482.html</a> .
Modification of an existing configuration starts with an
error "Error in device Picard Z Stage: Unable to communicate
with the device" followed by lots of COM and "No device with
label _device name_" -errors. Creating a new configuration
doesn't help since it is not possible to add Picard stage
z-axis to the config file or modify the serial number.
</p>
<p><br>
</p>
<p>We've tried to copy the latest Picard .dll-files from the
recent Micro-Manager nightly build but the problem
persists. Are there some other files we should copy and
replace as well? Of course we can still download and
configure the non-updated package from openspim.org and be
happy using that older version without any problems. Still
it would be nice to be up-to-date. Anyone having the same
problems or some solutions? Thanks!</p>
<p><br>
</p>
<p>Regards,</p>
<p>Veli-Pekka Ronkainen</p>
<p>Light Microscopy Core Facility </p>
<p>Biocenter Oulu, University of Oulu</p>
<p>Finland<br>
</p>
<p><br>
</p>
</div>
</div>
<br>
<fieldset class="mimeAttachmentHeader"></fieldset>
<br>
<pre wrap="">_______________________________________________
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</pre>
</blockquote>
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