<html><head><meta http-equiv="Content-Type" content="text/html charset=windows-1252"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><div>Hi Michael,</div><div><br></div><div>creating two separate light sheets has a couple of advantages. You are more flexible in setting up the system, and you can compensate better for potential deviations due to differences in lenses etc. I fear that creating a single light sheet and splitting it afterwards can be difficult to set up and align, because you have to consider the length of your optical paths. And the required additional splitters and mirrors potentially introduce aberrations.</div><div><br></div><div>I just picked the shutter in the T-SPIM concept because they are small and there was a 3D model available… but I believe people already built T-SPIM, right?! Using a 50/50 splitter and two AOTF/AOM (or two diode lasers) is another option.</div><div><br></div><div>Modifying the chamber for more than two lenses is straight forward, I think Pete already has a 3D model for that?!</div><div><br></div><div>Best,</div><div>Michael</div><div><br></div><br><div><div>On Jun 23, 2014, at 5:08 AM, Michael Redd <<a href="mailto:Michael.Redd@hci.utah.edu">Michael.Redd@hci.utah.edu</a>> wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite">For those who have built a T-SPIM, I have a several questions. The first question is about creating the second illumination light sheet. The T-SPIM drawing in the OPENSPIM gallery, a mirror is placed near the laser that splits the light between two identical light paths each complete with cylindrical lenses etc.. My question is rather than creating two identical light paths with many optical components, why can't a the light sheet be created in a single light path and a splitting mirror be placed at the end prior to the 10x Olympus dipping lens and routed with mirrors to the back of the other 10x dipping lens. The second question concerns shuttering the laser. Does anyone have any shutters that they would recommend for use with a T-SPIM? Finally, does anyone have a digital plans for a T-SPIM sample chamber?<br><br>thanks<br><br>Michael Redd<br><br><br><br><br><br>_______________________________________________<br>OpenSPIM mailing list<br><a href="mailto:OpenSPIM@openspim.org">OpenSPIM@openspim.org</a><br>http://openspim.org/mailman/listinfo/openspim<br></blockquote></div><br><div apple-content-edited="true">
<span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; font-size: medium; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; font-size: medium; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">_____________<br><br>Michael Weber<br>PhD Student, Huisken lab<br>Max Planck Institute of Molecular Cell Biology and Genetics<br>Pfotenhauerstrasse 108, 01307 Dresden<br>Tel. 0049 351/2102837<br><br><a href="http://www.mpi-cbg.de/huisken">http://www.mpi-cbg.de/huisken</a></div></span></div></span></span>
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