<html><head><meta http-equiv="Content-Type" content="text/html charset=windows-1252"><base href="x-msg://2765/"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">Dear Douglas,<div><br></div><div>This is what we do as well. Its not perfect, but it works. I guess we should document it on the wiki, but better Fiji then OpenSPIM since this is a LZ1 specific issue.</div><div><br></div><div>I have something like that here, but it deals exclusively with headless operation on a cluster <a href="http://fiji.sc/SPIM_Registration_on_cluster">http://fiji.sc/SPIM_Registration_on_cluster</a></div><div><br></div><div>Sending data to bioformats is a great idea, they are working on it.</div><div><br></div><div>All the best</div><div><br></div><div>PAvel</div><div><br><div>
<div style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><div style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><div><div style="font-size: 12px; ">-----------------------------------------------------------------------------------<br>Pavel Tomancak, Ph.D.<br><br>Group Leader<br>Max Planck Institute of Molecular Cell Biology and Genetics<br>Pfotenhauerstr. 108<br>D-01307 Dresden<span class="Apple-tab-span" style="white-space: pre; "> </span><span class="Apple-tab-span" style="white-space: pre; "> </span><span class="Apple-tab-span" style="white-space: pre; "> </span><span class="Apple-tab-span" style="white-space: pre; "> </span>Tel.: +49 351 210 2670<br>Germany<span class="Apple-tab-span" style="white-space: pre; "> </span><span class="Apple-tab-span" style="white-space: pre; "> </span><span class="Apple-tab-span" style="white-space: pre; "> </span><span class="Apple-tab-span" style="white-space: pre; "> </span><span class="Apple-tab-span" style="white-space: pre; "> </span><span class="Apple-tab-span" style="white-space: pre; "> </span>Fax: +49 351 210 2020<br>email: <a href="mailto:tomancak@mpi-cbg.de">tomancak@mpi-cbg.de</a><br>homepage: <a href="http://www.mpi-cbg.de/research/research-groups/pavel-tomancak.html">http://www.mpi-cbg.de/research/research-groups/pavel-tomancak.html</a></div><div style="font-size: 12px; ">twitter: @PavelTomancak<br>-----------------------------------------------------------------------------------</div></div><div><br></div></div><br class="Apple-interchange-newline"></div><br class="Apple-interchange-newline"><br class="Apple-interchange-newline">
</div>
<br><div><div>On Jun 16, 2014, at 5:32 PM, "Richardson, Douglas" <<a href="mailto:drichardson@fas.harvard.edu">drichardson@fas.harvard.edu</a>> wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"><div lang="EN-US" link="blue" vlink="purple" style="font-family: Helvetica; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><div class="WordSection1" style="page: WordSection1; "><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">Here is what I have found to work with our Z1 data:<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "> </span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">The newest version of Bioformats can read and open the .CZI file off the microscope. As Bioformats opens the file, it opens each view as a separate stack and gives you the option of which ones you want to open. As long as your system has enough RAM, you can open individual, or all views, and then convert to .tif using Fiji. If any of the views exceed your RAM, you will have to open them as a virtual stack, convert to .tif and downsample before running the registration/fusion plugins.<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "> </span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">If your data was subsetted in ZEN by anything other than time (channel/view/etc) during acquisition, it will not open in Bioformats.<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">Bioformats still seems to have problems reading certain multi-channel files. I have sent them some test data sets, but haven’t heard back yet.<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "> </span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">When all-else fails, the following protocol seems to work:<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "> </span></div><div style="margin: 0in 0in 0.0001pt 0.5in; font-size: 12pt; font-family: 'Times New Roman', serif; text-indent: -0.25in; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "><span>1)<span style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-family: 'Times New Roman'; "> <span class="Apple-converted-space"> </span></span></span></span><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">Subset your original file into individual .czi files for channel and view (don’t worry about time point). Do this using the Processing<Copy<File Subset command in ZEN, NOT File<New File Browser.<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt 0.5in; font-size: 12pt; font-family: 'Times New Roman', serif; text-indent: -0.25in; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "><span>2)<span style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-family: 'Times New Roman'; "> <span class="Apple-converted-space"> </span></span></span></span><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">You can then batch convert these .CZI files to .tif files using Fiji.<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt 0.5in; font-size: 12pt; font-family: 'Times New Roman', serif; text-indent: -0.25in; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "><span>3)<span style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-family: 'Times New Roman'; "> <span class="Apple-converted-space"> </span></span></span></span><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">These files can then be registered/fused/deconvolved<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "> </span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">If anyone has other/better suggestions, I’d be happy to hear them.<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "> </span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">-Doug<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "> </span></div><div><div style="border-style: solid none none; border-top-width: 1pt; border-top-color: rgb(181, 196, 223); padding: 3pt 0in 0in; "><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><b><span style="font-size: 10pt; font-family: Tahoma, sans-serif; ">From:</span></b><span style="font-size: 10pt; font-family: Tahoma, sans-serif; "><span class="Apple-converted-space"> </span><a href="mailto:openspim-bounces@openspim.org">openspim-bounces@openspim.org</a> [mailto:openspim-<a href="mailto:bounces@openspim.org">bounces@openspim.org</a>]<span class="Apple-converted-space"> </span><b>On Behalf Of<span class="Apple-converted-space"> </span></b>Selchow, Olaf<br><b>Sent:</b><span class="Apple-converted-space"> </span>Sunday, June 15, 2014 12:35 PM<br><b>To:</b><span class="Apple-converted-space"> </span>Kieran Short; <a href="mailto:Shugi@gmx.de">Shugi@gmx.de</a><br><b>Cc:</b><span class="Apple-converted-space"> </span><a href="mailto:openspim@openspim.org">openspim@openspim.org</a><br><b>Subject:</b><span class="Apple-converted-space"> </span>Re: [OpenSPIM] Input data for SPIM Registration PlugIn<o:p></o:p></span></div></div></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span lang="DE" style="font-size: 10pt; font-family: Arial, sans-serif; ">Dear Kieran and Ricarda,<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span lang="DE" style="font-size: 10pt; font-family: Arial, sans-serif; "> </span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 10pt; font-family: Arial, sans-serif; ">To my knowledge, the export of .czi data from the ZEISS Lightsheet Z.1 should be not necessary any more.<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 10pt; font-family: Arial, sans-serif; ">The .czi data can be read directly by Bioformats reader into Fiji …<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 10pt; font-family: Arial, sans-serif; "> </span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 10pt; font-family: Arial, sans-serif; ">Kevin Eliceiri and Stephan Preibisch are the experts in this …<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 10pt; font-family: Arial, sans-serif; "> </span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 10pt; font-family: Arial, sans-serif; ">Pls let me know if I can help in that data from ZEISS microscopes can be opened in the software where you need it.<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 10pt; font-family: Arial, sans-serif; "> </span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 10pt; font-family: Arial, sans-serif; ">With best regards,<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 10pt; font-family: Arial, sans-serif; ">Olaf<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 10pt; font-family: Arial, sans-serif; "> </span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span lang="DE" style="font-size: 9pt; font-family: Calibri, sans-serif; ">__________________________<br><br>Carl Zeiss Microscopy GmbH<br>Geschäftsbereich BioSciences / BioSciences Division<br>Produktmanager / Product Manager<br><br>D r. O l a f S e l c h o w<br><br>Carl-Zeiss-Promenade 10, 07745 Jena<br>Telefon/Phone: +49 3641 64-2089<br>Cell Phone: +49 151 4615 8629<br>Fax: +49 3641 64-3144<br></span><span lang="DE" style="font-size: 10pt; font-family: Tahoma, sans-serif; "><a href="mailto:olaf.selchow@zeiss.com" target="_blank" style="color: purple; text-decoration: underline; "><span style="font-size: 9pt; font-family: Calibri, sans-serif; color: purple; ">mailto:olaf.selchow@zeiss.com</span></a></span><span lang="DE" style="font-size: 9pt; font-family: Calibri, sans-serif; "><br></span><span lang="DE" style="font-size: 10pt; font-family: Tahoma, sans-serif; "><a href="http://www.zeiss.com/microscopy" target="_blank" style="color: purple; text-decoration: underline; "><span style="font-size: 9pt; font-family: Calibri, sans-serif; color: purple; ">http://www.zeiss.com/microscopy</span></a></span><span lang="DE" style="font-size: 9pt; font-family: Calibri, sans-serif; "><br><br>__________________________<br><br>Carl Zeiss Microscopy GmbH<br>Vorsitzender des Aufsichtsrates: Dr. Michael Kaschke <br>Geschäftsführung: Dr. Ulrich Simon, Wilhelm Nörthemann, Dr. Frank Stietz<br>Sitz der Gesellschaft: 07740 Jena, Deutschland <br>Amtsgericht Jena, HRB 210.536, USt-IdNr: DE 814 503 774</span><span lang="DE" style="font-size: 10pt; font-family: Tahoma, sans-serif; "><o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span lang="DE"> </span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><b><span lang="DE" style="font-size: 10pt; font-family: Tahoma, sans-serif; ">Von:</span></b><span lang="DE" style="font-size: 10pt; font-family: Tahoma, sans-serif; "><span class="Apple-converted-space"> </span><a href="mailto:openspim-bounces@openspim.org" style="color: purple; text-decoration: underline; ">openspim-bounces@openspim.org</a><span class="Apple-converted-space"> </span>[<a href="mailto:openspim-bounces@openspim.org" style="color: purple; text-decoration: underline; ">mailto:openspim-bounces@openspim.org</a>]<span class="Apple-converted-space"> </span><b>Im Auftrag von<span class="Apple-converted-space"> </span></b>Kieran Short<br><b>Gesendet:</b><span class="Apple-converted-space"> </span>Dienstag, 27. Mai 2014 23:19<br><b>An:</b><span class="Apple-converted-space"> </span><a href="mailto:Shugi@gmx.de" style="color: purple; text-decoration: underline; ">Shugi@gmx.de</a><br><b>Cc:</b><span class="Apple-converted-space"> </span><a href="mailto:openspim@openspim.org" style="color: purple; text-decoration: underline; ">openspim@openspim.org</a><br><b>Betreff:</b><span class="Apple-converted-space"> </span>Re: [OpenSPIM] Input data for SPIM Registration PlugIn<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span lang="DE"> </span></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span lang="DE">Richard,<o:p></o:p></span></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span lang="DE"> </span></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span lang="DE">The endianness of the TIFF data might be wrong (wrong byte order). Try switching to intel-byte order (or if it already is, switch it to big endian).<o:p></o:p></span></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span lang="DE"> </span></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span lang="DE">fyi: <a href="http://en.wikipedia.org/wiki/Endianness" style="color: purple; text-decoration: underline; ">http://en.wikipedia.org/wiki/Endianness</a><o:p></o:p></span></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span lang="DE"> </span></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span lang="DE">cheers,<o:p></o:p></span></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span lang="DE">Kieran<o:p></o:p></span></div></div></div><div><p class="MsoNormal" style="margin: 0in 0in 12pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span lang="DE"> </span></p><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span lang="DE">On Tue, May 27, 2014 at 8:19 PM, <<a href="mailto:Shugi@gmx.de" target="_blank" style="color: purple; text-decoration: underline; ">Shugi@gmx.de</a>> wrote:<o:p></o:p></span></div><div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span lang="DE" style="font-size: 9pt; font-family: Verdana, sans-serif; ">Hello,<o:p></o:p></span></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span lang="DE" style="font-size: 9pt; font-family: Verdana, sans-serif; "> <o:p></o:p></span></div></div><div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span lang="DE" style="font-size: 9pt; font-family: Verdana, sans-serif; ">I read about your tool for registration and fusion of multiple view images. It is the topic of my intership to find a way doing this with our data and I would like to try your tool. But it seems to have a problem with our data. The original data from a Z1 are present as czi file. I extract them as ome.tif file with the ZEN2012 Software. Like in the tutorial the extension was changed from ome.tif to tif. I was following the tutorial and when to interactivlly choosing the threshold for the beads and click "Done" the following Messages occure in the Log:<o:p></o:p></span></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span lang="DE" style="font-size: 9pt; font-family: Verdana, sans-serif; "> <o:p></o:p></span></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span lang="DE" style="font-size: 9pt; font-family: Verdana, sans-serif; ">Opening first image to determine z-stretching.<br>Cannot open fie: ...<o:p></o:p></span></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span lang="DE" style="font-size: 9pt; font-family: Verdana, sans-serif; "> <o:p></o:p></span></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span lang="DE" style="font-size: 9pt; font-family: Verdana, sans-serif; ">The path and the filename are correct. Maybe it is something about the formate? Do you need special tags in the tiff file or does the bit depth has influence?<o:p></o:p></span></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span lang="DE" style="font-size: 9pt; font-family: Verdana, sans-serif; ">Hope you can help me, because your tool seems to be very promising.<o:p></o:p></span></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span lang="DE" style="font-size: 9pt; font-family: Verdana, sans-serif; "> <o:p></o:p></span></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span lang="DE" style="font-size: 9pt; font-family: Verdana, sans-serif; ">Kind regardas<o:p></o:p></span></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span lang="DE" style="font-size: 9pt; font-family: Verdana, sans-serif; ">Ricarda Bräuniger<o:p></o:p></span></div></div></div></div><p class="MsoNormal" style="margin: 0in 0in 12pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span lang="DE"><br>_______________________________________________<br>OpenSPIM mailing list<br><a href="mailto:OpenSPIM@openspim.org" style="color: purple; text-decoration: underline; ">OpenSPIM@openspim.org</a><br><a href="http://openspim.org/mailman/listinfo/openspim" target="_blank" style="color: purple; text-decoration: underline; ">http://openspim.org/mailman/listinfo/openspim</a><o:p></o:p></span></p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><span lang="DE"> </span></div></div></div>_______________________________________________<br>OpenSPIM mailing list<br><a href="mailto:OpenSPIM@openspim.org">OpenSPIM@openspim.org</a><br>http://openspim.org/mailman/listinfo/openspim</div></blockquote></div><br></div></body></html>