<html><head></head><body><div style="font-family: Verdana;font-size: 12.0px;"><div>Hello,</div>
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<div>I read about your tool for registration and fusion of multiple view images. It is the topic of my intership to find a way doing this with our data and I would like to try your tool. But it seems to have a problem with our data. The original data from a Z1 are present as czi file. I extract them as ome.tif file with the ZEN2012 Software. Like in the tutorial the extension was changed from ome.tif to tif. I was following the tutorial and when to interactivlly choosing the threshold for the beads and click "Done" the following Messages occure in the Log:</div>
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<div>Opening first image to determine z-stretching.<br/>
Cannot open fie: ...</div>
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<div>The path and the filename are correct. Maybe it is something about the formate? Do you need special tags in the tiff file or does the bit depth has influence?</div>
<div>Hope you can help me, because your tool seems to be very promising.</div>
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<div>Kind regardas</div>
<div>Ricarda Bräuniger</div>
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