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<span>Hi Luke,<br><br>I will send you all the information requested on monday, since now I am not at the<br>
institute. Hopefully with the no laser patch the problem could be solved, but we have<br>
to try first. <br><br>About the "Stack at this Z plus" button, it is the only problem, it looks that it sticks. I can still<br>
move the stage, change the tabs, add a new single position in the position list, use snap<br>
images and save them in RAM or directly in disk. In resume, I can do everything except set a <br>
stack of positions in the position list.<br><br>Thanks to you!! and see you on monday.<br>
<br>Kind regards,<br>Edgar<br><table border="0" cellpadding="0" cellspacing="0" width="457,5">
<tbody><tr><td colspan="3"><font face="sans-serif" size="2">_________________________________________________________________________<br>
<br>Fraunhofer-Institut für Produktionstechnologie IPT <br>Edgar Escobar Nieto <br>
<br><br> <br> <br>Steinbachstraße 17 <br>52074 Aachen <br><br>edgar.escobar.nieto@ipt.fraunhofer.de </font>
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<font color="blue" face="sans-serif" size="2"><u>http://www.ipt.fraunhofer.de</u>
</font></a><font face="sans-serif" size="2"><br>_________________________________________________________________________</font>
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<br><br><font color="#990099">-----Luke Stuyvenberg <stuyvenberg@wisc.edu> schrieb: -----</font>
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An: edgar.escobar.nieto@ipt.fraunhofer.de<br>Von: Luke Stuyvenberg <stuyvenberg@wisc.edu><br>
Datum: 25.10.2013 20:14<br>Kopie: openspim@openspim.org<br>Betreff: Re: "Stack at this Z plus" problem persisting<br>
<br><div><div>Hi Edgar,</div><div><br></div><div>Please don't worry about disturbing me; it's what I am here for, after all!</div>
<div><br></div><div>You are absolutely correct about the missing TIScam DLLs; I forgot to include them in the new version. I will head to LOCI and correct that in two hours or so.</div>
<div><br></div><div>I'm still at a loss with regards to the errors you are encountering, however. I will have a patch for the 'no laser' problem early next week, hopefully alongside the autostart fix, but until we can diagnose the acquisition bug, I won't know how to fix it.</div>
<div><br></div><div>With that in mind, I have some more information to request:</div>
<div>- Please attach to your reply all core logs, hs error files, and configuration files (your active one, in particular), compressed with your file archiver of choice.</div>
<div>- Please list a) all hardware involved in the setup (with model numbers, if you have them), and b) all devices you set up in the hardware configuration.</div>
<div>- When you click the 'insert stack' button, you say it sticks down and nothing happens -- is the rest of the UI still responsive? Can you change tabs? Move the stage?</div>
<div><br></div><div>I'm casting a pretty wide net, here, but hopefully somewhere in there will be a vital clue.</div>
<div><br></div><div>Thanks,</div><div>Luke</div></div><br><br>edgar.escobar.nieto@ipt.fraunhofer.de wrote:<br>
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Dear <span>Luke,<br><br>I am sorry to disturb you again but I have been struggling a lot with SPIM acquisiton plugin :(. Hopefully I made some discoveries.<br>
<br>I updated Fiji and again had the problem again about the TIScam (The Imaging Source camera). But I discover that <br>
<b>mmgr_dal_TIScam.dll</b> depends on <b>TIS_DShowLib10.dll</b> and <b>
TIS_UDSHL10.dll</b> but in the mm\Win32 location are<br><b>TIS_UDSHL09_vc9d.dll</b>
and <b>TIS_DShowLib09.dll</b>. I got the version 10 of the DLLs from the 1.14.16 version of a plain <br>
installation of MicroManager and just copied them to </span><span><span>
mm\Win32. Maybe you can solve that from the Fiji updater: to uninstall <br>
the version 9 of the DLLs and to install the version 10. Anayway the problem about the camera is fixed now. I attach the<br>
version 10 of the DLLs to this mail.<br><br>About the "Stack at this Z plus" button I still have the problem when using the updated version of Fiji. I also discover that if I don't <br>
use the demo shuter I can't write the images direclty to disk, the message "Error acquiring: null" appears and in the output directory is saved a .ome.tiff<br>
empty image (0 byte). </span></span>S<span><span><span><span>o for the moment I have to use the no updated version of Fiji in order to perform SPIM acquisiton.<br>
<br></span></span>The problem about using the no updated version of Fiji is that the plugin don't respond when pressing "Oh Snap" (as<br>
it happens when pressing "Stack at this Z plus" in the Fiji updated version) when I want to write the images directly to disk.<br>
<br>So at the end if I want to perform SPIM image acquisition (the way I have done that until now), I have to use the no updated version <br>
of Fiji and save the images to RAM no specifying an ouput directory. The problem is that I am limited by the RAM and all the images<br>
are saved in the same stack even if different positions are specified in the position list.<br>
<br>Finally to be sure that the problem about the "Stack at this Z plus" button is not related to the computer I am using (Windows XP 32 bit)<br>
I tried Fiji in a different computer (Windows 7 64 bit).<br><br>I really would like to use the SPIM image acquisition plugin and benefit from all his capabilities.<br>
<br>Thanks in advance for your comments.<br><br>Kind regards,<br>Edgar<br>
<br><br><br><br><br></span><table border="0" cellpadding="0" cellspacing="0" width="457,5">
<tbody><tr><td colspan="3"><font face="sans-serif" size="2">_________________________________________________________________________<br>
<br>Fraunhofer-Institut für Produktionstechnologie IPT <br>Edgar Escobar Nieto <br>
<br><br> <br> <br>Steinbachstraße 17 <br>52074 Aachen <br><br>edgar.escobar.nieto@ipt.fraunhofer.de </font>
<font color="blue" size="3"><u><br></u></font><a href="http://www.ipt.fraunhofer.de/">
<font color="blue" face="sans-serif" size="2"><u>http://www.ipt.fraunhofer.de</u>
</font></a><font face="sans-serif" size="2"><br>_________________________________________________________________________</font>
<br></td></tr><tr><td valign="top" width="225"><br></td><td width="7,5">
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