<font face="Default Sans Serif,Verdana,Arial,Helvetica,sans-serif" size="2">
<span>Hi dear Peter,<br><br>I have not yet bought the Ronchi Ruler Slide, but I was thinking of use a different<br>
material in order to perform the alignment of the lighstsheet. I was thinking of use<br>
some fluorescence samples that are mounted in a glass http://www.bw-optik.de/laufband/fluoreszenz.php<br>
and since I have already some of this mounted glass samples available I will try to make a similar procedure as the stated in the OpenSPIM wiki.<br>
If that method doesn't work I will have to buy the Ronchi Ruler Slide anyway.<br>
<br>The think is that since the material is also glass, would be the same to cut it or to cut the Ronchi Ruler Slide. That's<br>
why I was asking how the Ruler Slide was cut. I will try to find a way to cut the glass, the problem<br>
is that only a thin piece, let's say from 1 to 2 mm wide is needed. I know that would be so difficul to perform a cut<br>
of this magnitude using a conventional glass cutter, but I'll give a try, if I am successful I will write a protocol to the OpenSPIM wiki.<br>
<br>I think until I had all the necessary material ready and mounted and if I have troubles with the alignment of the lightsheet,<br>
that would be nice to have the Skype session.<br><br>Thanks for your kind answer and for offering me your support. Enjoy your holidays in the U.S.<br>
<br>Kind regards,<br>Edgar<br><table border="0" cellpadding="0" cellspacing="0" width="457,5">
<tbody><tr><td colspan="3"><font face="sans-serif" size="2">_________________________________________________________________________<br>
<br>Fraunhofer-Institut für Produktionstechnologie IPT <br>Edgar Escobar Nieto <br>
<br><br> <br> <br>Steinbachstraße 17 <br>52074 Aachen <br><br>edgar.escobar.nieto@ipt.fraunhofer.de </font>
<font color="blue" size="3"><u><br></u></font><a href="http://www.ipt.fraunhofer.de/">
<font color="blue" face="sans-serif" size="2"><u>http://www.ipt.fraunhofer.de</u>
</font></a><font face="sans-serif" size="2"><br>_________________________________________________________________________</font>
<br></td></tr><tr><td valign="top" width="225"><br></td><td width="7,5">
<br></td><td valign="top" width="225"><br></td></tr></tbody></table></span>
<br><br><font color="#990099">-----"Peter Gabriel Pitrone" <pitrone@mpi-cbg.de> schrieb: -----</font>
<div style="padding-left:5px;"><div style="padding-right:0px;padding-left:5px;border-left:solid black 2px;">
An: edgar.escobar.nieto@ipt.fraunhofer.de<br>Von: "Peter Gabriel Pitrone" <pitrone@mpi-cbg.de><br>
Datum: 06.08.2013 18:22<br>Kopie: openspim@openspim.org<br>Betreff: Re: [OpenSPIM] About the calibration of the lightsheet<br>
<br><div><font face="Courier New,Courier,monospace" size="2">Hello Edgar,<br>
<br>I'm sorry I didn't answer you till now, but I'm sometimes away from my<br>
email as I am on vacation in the states.<br><br>To answer your questions, we broke the slide and found the best shard. It<br>
is definitely not the most elegant way, but it works for us. If you want<br>
to go about making it with more consistency with a glass or window<br>cutter, please write a protocol on our wiki on how to do it so others can<br>
reproduce it.<br><br>If you need help with aligning the light sheet, we can make a skype<br>
session tomorrow... just remember I have an 8 hour difference in<br>timezones. My Skype name is "petepitrone". send me an email as to the<br>
time you would like to talk.<br><br>Pete<br><br>-- <br>Peter Gabriel Pitrone - TechRMS<br>
Microscopy/Imaging Specialist<br>Prof. Dr. Pavel Tomancak group<br>Max Planck Institute for<br>
Molecular Biology and Genetics<br>Pfotenhauerstr. 108<br>01307 Dresden<br>
<br>"If a straight line fit is required, obtain only two data points." - Anon.<br>
<br><br>On Tue, August 6, 2013 5:08 pm, edgar.escobar.nieto@ipt.fraunhofer.de wrote:<br>
<|> Hi dear All,<br><|><br><|> I would like to know how you could cut the ruling slides. I don't<br>
know if<br><|> it is possible tu cut a thin part of glass using<br>
<|> a conventional glass cutter.<br><|><br><|> Kind regards,<br>
<|> Edgar Escobar Nieto<br><|> <br>_________________________________________________________________________<br>
<|><br><|> Fraunhofer-Institut für Produktionstechnologie IPT<br>
<|> Edgar Escobar Nieto<br><|><br><|><br><|><br>
<|><br><|> Steinbachstraße 17<br><|> 52074 Aachen<br>
<|><br><|> edgar.escobar.nieto@ipt.fraunhofer.de<br><|> <a href="http://www.ipt.fraunhofer.de">
http://www.ipt.fraunhofer.de</a><br><|><br>_________________________________________________________________________<br>
<|><br><|><br><|><br><|><br><|><br><|> -----Weitergeleitet von Edgar Escobar Nieto/Fraunhofer IPT am 06.08.2013<br>
<|> 17:01 -----<br><|> An: openspim@openspim.org<br><|> Von: Edgar Escobar Nieto/Fraunhofer IPT<br>
<|> Datum: 05.08.2013 11:55<br><|> Betreff: About the calibration of the lightsheet<br>
<|><br><|><br><|> <br>_________________________________________________________________________<br>
<|><br><|> Fraunhofer-Institut für Produktionstechnologie IPT<br>
<|> Edgar Escobar Nieto<br><|><br><|><br><|><br>
<|><br><|> Steinbachstraße 17<br><|> 52074 Aachen<br>
<|><br><|> edgar.escobar.nieto@ipt.fraunhofer.de<br><|> <a href="http://www.ipt.fraunhofer.de">
http://www.ipt.fraunhofer.de</a><br><|><br>_________________________________________________________________________<br>
<|><br><|><br><|><br><|><br><|><br><|> -----Weitergeleitet von Edgar Escobar Nieto/Fraunhofer IPT am 05.08.2013<br>
<|> 11:54 -----<br><|> An: openspim@openspim.org<br><|> Von: Edgar Escobar Nieto/Fraunhofer IPT<br>
<|> Datum: 31.07.2013 15:02<br><|> Betreff: (Unbenannt)<br>
<|><br><|> Hi dear all,<br><|><br><|> I reached to the point where I need to calibrate the light-sheet. And I<br>
<|> was wondering<br><|> what is the optical density of the ND filter recommended to perform the<br>
<|> calibration.<br><|><br><|> I would like to know also what is the Frequency (lp/mm) recommended for<br>
<|> the Opal Glass Ronchi Ruling Slides.<br><|> I think that high a frequency would be better, but there must be a<br>
limit.<br><|><br><|> Only to be sure, what I understood is that these Ruling Slides have<br>
<|> already a reflective surface, so there is no<br><|> need to glue a mirror to the ruling slides.<br>
<|><br><|> Thank you in advance for your comments.<br><|><br>
<|> Kind regards,<br><|> Edgar Escobar Nieto<br><|> <br>
_________________________________________________________________________<br>
<|><br><|> Fraunhofer-Institut für Produktionstechnologie IPT<br>
<|> Edgar Escobar Nieto<br><|><br><|><br><|><br>
<|><br><|> Steinbachstraße 17<br><|> 52074 Aachen<br>
<|><br><|> edgar.escobar.nieto@ipt.fraunhofer.de<br><|> <a href="http://www.ipt.fraunhofer.de">
http://www.ipt.fraunhofer.de</a><br><|><br>_________________________________________________________________________<br>
<|><br><|><br><|><br><|><br><|><br><|> _______________________________________________<br>
<|> OpenSPIM mailing list<br><|> OpenSPIM@openspim.org<br><|> <a href="http://openspim.org/mailman/listinfo/openspim">
http://openspim.org/mailman/listinfo/openspim</a><br><|><br><br><br>
</font></div></div></div><div></div></font>