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<span>Hi Jan,<br><br>Thanks a lot for your comments. <br>I will search in the lab if there is a little mirror, otherwise I should buy it.<br>
The method that you used is also very clever. That will be the one that I will<br>
use since looks like it is cheaper to buy a little simple mirror.<br><br>
Kind regards,<br>Edgar<br><br><table border="0" cellpadding="0" cellspacing="0" width="457,5">
<tbody><tr><td colspan="3"><font face="sans-serif" size="2">_________________________________________________________________________<br>
<br>Fraunhofer-Institut für Produktionstechnologie IPT <br>Edgar Escobar Nieto <br>
<br><br> <br> <br>Steinbachstraße 17 <br>52074 Aachen <br><br>edgar.escobar.nieto@ipt.fraunhofer.de </font>
<font color="blue" size="3"><u><br></u></font><a href="http://www.ipt.fraunhofer.de/">
<font color="blue" face="sans-serif" size="2"><u>http://www.ipt.fraunhofer.de</u>
</font></a><font face="sans-serif" size="2"><br>_________________________________________________________________________</font>
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<br><br><font color="#990099">-----"Krieger, Jan" <j.krieger@Dkfz-Heidelberg.de> schrieb: -----</font>
<div style="padding-left:5px;"><div style="padding-right:0px;padding-left:5px;border-left:solid black 2px;">
An: "edgar.escobar.nieto@ipt.fraunhofer.de" <edgar.escobar.nieto@ipt.fraunhofer.de><br>
Von: "Krieger, Jan" <j.krieger@Dkfz-Heidelberg.de><br>Datum: 07.08.2013 12:21<br>
Betreff: AW: [OpenSPIM] About the calibration of the lightsheet<br><br>
<div><font face="Courier New,Courier,monospace" size="2">Hi!<br><br>we are using a simple but small silver mirror (5x5mm², 1mm thick, bought from Melles-Griot) on a special mount, but you can glue it to anything that can be mounted inside the SPIM. First we focus the mirror, by illuminating it with a white LED or sinple lamp through the projection objective. The mirror is small enough to also fit into an openSPIM (I did that together with Alexis Maizel two weeks ago). Basically you can focus onto some dirk on the mirror surface, or a skratch that we make into it. Then you can switch to the laser and first bring it to the sharp line on the mirror and finally align it with the lenses of the illumination beam path. I think this is kind of the "classical" method to do that. <br>
<br>The samples seem to be embedded in glass, right? Then you can focus them, but you won't have a focussed reflective surface then, that you can use to inspect the lightsheet.<br>
<br>Best,<br>JAN<br><br><br>Dipl.-Phys. Jan Krieger<br>German Cancer Research Center (dkfz)<br>
Department B040 - Biophysics of Macromolecules (Prof. J. Langowski)<br>
Im Neuenheimer Feld 580<br>69120 Heidelberg<br><br>fon: +49 / 6221 / 42-3395<br>
fax: +49 / 6221 / 42-3391<br>e-mail: j.krieger@dkfz.de<br>www: <a href="http://www.dkfz.de/Macromol/">
http://www.dkfz.de/Macromol/</a><br>________________________________________<br>
Von: openspim-bounces@openspim.org [openspim-bounces@openspim.org] im Auftrag von edgar.escobar.nieto@ipt.fraunhofer.de [edgar.escobar.nieto@ipt.fraunhofer.de]<br>
Gesendet: Mittwoch, 7. August 2013 12:13<br>An: Peter Gabriel Pitrone<br>
Cc: openspim@openspim.org<br>Betreff: Re: [OpenSPIM] About the calibration of the lightsheet<br>
<br>Hi dear Peter,<br><br>I have not yet bought the Ronchi Ruler Slide, but I was thinking of use a different<br>
material in order to perform the alignment of the lighstsheet. I was thinking of use<br>
some fluorescence samples that are mounted in a glass <a href="http://www.bw-optik.de/laufband/fluoreszenz.php">
http://www.bw-optik.de/laufband/fluoreszenz.php</a><br>and since I have already some of this mounted glass samples available I will try to make a similar procedure as the stated in the OpenSPIM wiki.<br>
If that method doesn't work I will have to buy the Ronchi Ruler Slide anyway.<br>
<br>The think is that since the material is also glass, would be the same to cut it or to cut the Ronchi Ruler Slide. That's<br>
why I was asking how the Ruler Slide was cut. I will try to find a way to cut the glass, the problem<br>
is that only a thin piece, let's say from 1 to 2 mm wide is needed. I know that would be so difficul to perform a cut<br>
of this magnitude using a conventional glass cutter, but I'll give a try, if I am successful I will write a protocol to the OpenSPIM wiki.<br>
<br>I think until I had all the necessary material ready and mounted and if I have troubles with the alignment of the lightsheet,<br>
that would be nice to have the Skype session.<br><br>Thanks for your kind answer and for offering me your support. Enjoy your holidays in the U.S.<br>
<br>Kind regards,<br>Edgar<br>_________________________________________________________________________<br>
<br>Fraunhofer-Institut für Produktionstechnologie IPT<br>Edgar Escobar Nieto<br>
<br><br><br><br>Steinbachstraße 17<br>52074 Aachen<br><br>edgar.escobar.nieto@ipt.fraunhofer.de<br>
<a href="http://www.ipt.fraunhofer.de">http://www.ipt.fraunhofer.de</a>
<<a href="http://www.ipt.fraunhofer.de/">http://www.ipt.fraunhofer.de/</a>
><br>_________________________________________________________________________<br>
<br><br><br><br><br><br><br>-----"Peter Gabriel Pitrone" <pitrone@mpi-cbg.de> schrieb: -----<br>
An: edgar.escobar.nieto@ipt.fraunhofer.de<br>Von: "Peter Gabriel Pitrone" <pitrone@mpi-cbg.de><br>
Datum: 06.08.2013 18:22<br>Kopie: openspim@openspim.org<br>Betreff: Re: [OpenSPIM] About the calibration of the lightsheet<br>
<br>Hello Edgar,<br><br>I'm sorry I didn't answer you till now, but I'm sometimes away from my<br>
email as I am on vacation in the states.<br><br>To answer your questions, we broke the slide and found the best shard. It<br>
is definitely not the most elegant way, but it works for us. If you want<br>
to go about making it with more consistency with a glass or window<br>cutter, please write a protocol on our wiki on how to do it so others can<br>
reproduce it.<br><br>If you need help with aligning the light sheet, we can make a skype<br>
session tomorrow... just remember I have an 8 hour difference in<br>timezones. My Skype name is "petepitrone". send me an email as to the<br>
time you would like to talk.<br><br>Pete<br><br>--<br>Peter Gabriel Pitrone - TechRMS<br>
Microscopy/Imaging Specialist<br>Prof. Dr. Pavel Tomancak group<br>Max Planck Institute for<br>
Molecular Biology and Genetics<br>Pfotenhauerstr. 108<br>01307 Dresden<br>
<br>"If a straight line fit is required, obtain only two data points." - Anon.<br>
<br><br>On Tue, August 6, 2013 5:08 pm, edgar.escobar.nieto@ipt.fraunhofer.de wrote:<br>
<|> Hi dear All,<br><|><br><|> I would like to know how you could cut the ruling slides. I don't<br>
know if<br><|> it is possible tu cut a thin part of glass using<br>
<|> a conventional glass cutter.<br><|><br><|> Kind regards,<br>
<|> Edgar Escobar Nieto<br><|><br>_________________________________________________________________________<br>
<|><br><|> Fraunhofer-Institut für Produktionstechnologie IPT<br>
<|> Edgar Escobar Nieto<br><|><br><|><br><|><br>
<|><br><|> Steinbachstraße 17<br><|> 52074 Aachen<br>
<|><br><|> edgar.escobar.nieto@ipt.fraunhofer.de<br><|> <a href="http://www.ipt.fraunhofer.de">
http://www.ipt.fraunhofer.de</a><br><|><br>_________________________________________________________________________<br>
<|><br><|><br><|><br><|><br><|><br><|> -----Weitergeleitet von Edgar Escobar Nieto/Fraunhofer IPT am 06.08.2013<br>
<|> 17:01 -----<br><|> An: openspim@openspim.org<br><|> Von: Edgar Escobar Nieto/Fraunhofer IPT<br>
<|> Datum: 05.08.2013 11:55<br><|> Betreff: About the calibration of the lightsheet<br>
<|><br><|><br><|><br>_________________________________________________________________________<br>
<|><br><|> Fraunhofer-Institut für Produktionstechnologie IPT<br>
<|> Edgar Escobar Nieto<br><|><br><|><br><|><br>
<|><br><|> Steinbachstraße 17<br><|> 52074 Aachen<br>
<|><br><|> edgar.escobar.nieto@ipt.fraunhofer.de<br><|> <a href="http://www.ipt.fraunhofer.de">
http://www.ipt.fraunhofer.de</a><br><|><br>_________________________________________________________________________<br>
<|><br><|><br><|><br><|><br><|><br><|> -----Weitergeleitet von Edgar Escobar Nieto/Fraunhofer IPT am 05.08.2013<br>
<|> 11:54 -----<br><|> An: openspim@openspim.org<br><|> Von: Edgar Escobar Nieto/Fraunhofer IPT<br>
<|> Datum: 31.07.2013 15:02<br><|> Betreff: (Unbenannt)<br>
<|><br><|> Hi dear all,<br><|><br><|> I reached to the point where I need to calibrate the light-sheet. And I<br>
<|> was wondering<br><|> what is the optical density of the ND filter recommended to perform the<br>
<|> calibration.<br><|><br><|> I would like to know also what is the Frequency (lp/mm) recommended for<br>
<|> the Opal Glass Ronchi Ruling Slides.<br><|> I think that high a frequency would be better, but there must be a<br>
limit.<br><|><br><|> Only to be sure, what I understood is that these Ruling Slides have<br>
<|> already a reflective surface, so there is no<br><|> need to glue a mirror to the ruling slides.<br>
<|><br><|> Thank you in advance for your comments.<br><|><br>
<|> Kind regards,<br><|> Edgar Escobar Nieto<br><|><br>
_________________________________________________________________________<br>
<|><br><|> Fraunhofer-Institut für Produktionstechnologie IPT<br>
<|> Edgar Escobar Nieto<br><|><br><|><br><|><br>
<|><br><|> Steinbachstraße 17<br><|> 52074 Aachen<br>
<|><br><|> edgar.escobar.nieto@ipt.fraunhofer.de<br><|> <a href="http://www.ipt.fraunhofer.de">
http://www.ipt.fraunhofer.de</a><br><|><br>_________________________________________________________________________<br>
<|><br><|><br><|><br><|><br><|><br><|> _______________________________________________<br>
<|> OpenSPIM mailing list<br><|> OpenSPIM@openspim.org<br><|> <a href="http://openspim.org/mailman/listinfo/openspim">
http://openspim.org/mailman/listinfo/openspim</a><br><|><br><br><br>
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