<html><head><meta http-equiv="Content-Type" content="text/html charset=us-ascii"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><div>We have a Jove publication regarding fish mounting in the loop. Once it's published I can contribute a "fish mounting" section with the current embedding strategies. I will also work on a bead sample protocol.</div><div><br></div><div>Best,</div><div>Michael</div><div><br></div><br><div><div>On May 28, 2013, at 9:17 AM, Pavel Tomancak <<a href="mailto:tomancak@mpi-cbg.de">tomancak@mpi-cbg.de</a>> wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"><blockquote type="cite">also it would be good for us to have on the site, some good test samples that people could use for testing their spim system. Any ideas? Could describe simple fly or fish mount. But perhaps a fluorescent bead sample? <br></blockquote><br>We have an extremely detailed protocol for Drosophila sample preparation. Fish would be good of course.<br><br><blockquote type="cite"><br>Also would be good to have a example raw data set (for download) so folks can try SPIM registration.<br></blockquote><br>Yes, good idea, I will include it into my SPIMage processing tutorial.<br><br>All the best<br><br>PAvel<br><br><blockquote type="cite"><br><br>best<br>k<br><br>--<br>Kevin W. Eliceiri<br>Director, Laboratory for Optical and Computational Instrumentation (LOCI)<br>Departments Cell and Molecular Biology and Biomedical Engineering<br>Affiliate Principal Investigator, Morgridge Institute for Research (MIR)<br>Room 271 Animal Sciences, 1675 Observatory Drive, Madison, WI 53706<br>Phone: 608-263-6288<br><br>_______________________________________________<br>OpenSPIM mailing list<br><a href="mailto:OpenSPIM@openspim.org">OpenSPIM@openspim.org</a><br>http://openspim.org/mailman/listinfo/openspim<br></blockquote><br><br>_______________________________________________<br>OpenSPIM mailing list<br><a href="mailto:OpenSPIM@openspim.org">OpenSPIM@openspim.org</a><br>http://openspim.org/mailman/listinfo/openspim<br></blockquote></div><br><div apple-content-edited="true">
<span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; font-size: medium; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; font-size: medium; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">_____________<br><br>Michael Weber<br>PhD Student, Huisken lab<br>Max Planck Institute of Molecular Cell Biology and Genetics<br>Pfotenhauerstrasse 108, 01307 Dresden<br>Tel. 0049 351/2102837<br><br><a href="http://www.mpi-cbg.de/huisken">http://www.mpi-cbg.de/huisken</a></div></span></div></span></span>
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