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<div>Hello Peter,</div><div><br></div><div>I will use the Laser, however it is not portable, it is a very good illumination source. I think I won't need filter for excitation</div>
<div>since this laser is of variable wavelength and has accesories like variable and tunable filters. About the emission filter it is a</div>
<div>good idea to have a quad or <span style="font-family: verdana, helvetica, arial, sans-serif;">
penta band filter. Right now I have a band pass filter especially for GFP intended for the first</span>
</div><div><span style="font-family: verdana, helvetica, arial, sans-serif;">
experiments.</span></div><div><span style="font-family: verdana, helvetica, arial, sans-serif;">
<br></span></div><div><span style="font-family: verdana, helvetica, arial, sans-serif;">
Kind regards,</span></div><div><span style="font-family: verdana, helvetica, arial, sans-serif;">
Edgar</span></div><div><table border="0" cellpadding="0" cellspacing="0" width="457,5">
<tbody><tr><td colspan="3"><font size="2" face="sans-serif">_________________________________________________________________________<br>
<br>Fraunhofer-Institut für Produktionstechnologie IPT <br>Edgar Escobar Nieto <br>
<br><br> <br> <br>Steinbachstraße 17 <br>52074 Aachen <br><br>skf-ee@ipt.rwth-aachen.de </font>
<font size="3" color="blue"><u><br></u></font><a href="http://www.ipt.fraunhofer.de/">
<font size="2" color="blue" face="sans-serif"><u>http://www.ipt.fraunhofer.de</u>
</font></a><font size="2" face="sans-serif"><br>_________________________________________________________________________</font>
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-----"Peter Gabriel Pitrone" <pitrone@mpi-cbg.de> schrieb: -----</font>
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An: skf-ee@ipt.rwth-aachen.de<br>Von: "Peter Gabriel Pitrone" <pitrone@mpi-cbg.de><br>
Datum: 22.05.2013 16:10<br>Kopie: openspim@openspim.org<br>Betreff: Re: [OpenSPIM] About the illumination source (laser)<br>
<br><div><font face="Courier New,Courier,monospace" size="3">Hello Edgar,<br>
<br>Get the laser!! I've wanted to do this for a while: all you need are normal fluorescent filters on<br>
a six position filter wheel to filter out the light for excitation, and a Quad or Penta Emission<br>
filter. You would then have an extremely versatile imaging system.<br><br>
My $0.02<br><br>Have a good one!!<br>Pete<br><br>-- <br>Peter Gabriel Pitrone - TechRMS<br>
Microscopy/Imaging Specialist<br>Prof. Dr. Pavel Tomancak group<br>Max Planck Institute for<br>
Molecular Biology and Genetics<br>Pfotenhauerstr. 108<br>01307 Dresden<br>
<br>"If a straight line fit is required, obtain only two data points." - Anon.<br>
<br><br>On Wed, May 22, 2013 12:35 pm, skf-ee@ipt.rwth-aachen.de wrote:<br>
<|> Hi to all,<br><|><br><|> I am having some options and at the same time some problems about the illumination source for the<br>
<|> SPIM system.<br><|><br><|> The firs option is a CoolLED pE-1 system<br>
<|> <a href="http://www.coolled.com/Life-Sciences-Analytical/Products/pE-1/">
http://www.coolled.com/Life-Sciences-Analytical/Products/pE-1/</a> with a Led of 470 nm wavelength,<br>
<|> the problem of this illumination system is that the output light is not very well collimated, and<br>
<|> the diamater of the light is so big. I have found out that there is a fiber adaptor<br>
<|> <a href="http://www.coolled.com/Life-Sciences-Analytical/Products/pE-100F/">
http://www.coolled.com/Life-Sciences-Analytical/Products/pE-100F/</a> for this system. My question is<br>
<|> that if after<br><|> the fiber, the light will be as collimated and coherent as a laser source? The good thing is that<br>
<|> this system<br><|> is already supported by µManager.<br>
<|><br><|> The second option is a NKT Photonics SuperK EXTREME<br>
<|> <a href="http://www.nktphotonics.com/superkextreme?cid=7858">
http://www.nktphotonics.com/superkextreme?cid=7858</a>, this one is a very good laser source, but the<br>
<|> problem is that it is not supported by µManager.<br><|><br>
<|> Have somebody had some issues like the ones I am having?<br><|> Thank you very much in advance, suggestions are welcome.<br>
<|><br><|> Edgar Escobar Nieto<br><|> _________________________________________________________________________<br>
<|><br><|> Fraunhofer-Institut für Produktionstechnologie IPT<br>
<|> Edgar Escobar Nieto<br><|><br><|><br><|><br>
<|><br><|> Steinbachstraße 17<br><|> 52074 Aachen<br>
<|><br><|> skf-ee@ipt.rwth-aachen.de<br><|> <a href="http://www.ipt.fraunhofer.de">
http://www.ipt.fraunhofer.de</a><br><|><br>________________________________________________________________________________________________________________________<br>
<|> OpenSPIM mailing list<br><|> OpenSPIM@openspim.org<br><|> <a href="http://openspim.org/mailman/listinfo/openspim">
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