User:Emese

Emese Palfi is a participant of the EMBO practical course Light sheet microscopy 2014.

Project
I am a PhD student at Semmelweis University in Budapest. Our goal is to understand the cortical mechanisms of tactile perception and skillful manual functions based on the information originating from the fingertips. To this end we combine intrinsic signal optical imaging, electrophysiological mapping, tract tracing and multiple immunolabeling techniques in primates (Samiri sciureus and Macaca mulatta).



Our future plan is to apply clearing techniques (CLARITY, ClearT or SeeDB) and light sheet microscopy as these techniques would boost our research by decreasing the number of histological sections used for reconstruction of labeling.

Skills:
 * immunhistochemistry
 * standard light microscopy
 * confocal microscopy



Goal at the course
At the course we can investigate one tracer BDA and three types of GABAergic interneurons (calretinin, parvalbumin and somatostatin) in the suqirrel monkey cortex, and it is a good complement to my confocal microscope experiments. We can answer a lot of questions:
 * does the clearing working on adult primate corte?
 * how deep can the the antibodies penetrate?
 * have data about the distriburion of GABAergic interneurons and tracer labeled cells and axons in the motor and somatosensory cortex of primates.

Microscopes used at the course

 * LSM Z1
 * Ultramicroscope