Team Clarity

Mice development
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Branching organs
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3D cultures
Mouse embryonic kidneys at E11.5, E12 and E12.5 were imaged with Zeiss Lightsheet Z.1. Light sheet microscopy provides cellular resolution at the organ scale. Images are coming soon.

Live samples
Colonies of Membranipora membranacea were shipped twice from Bergen, Norway. In both cases colonies arrived dead or mostly-dead. However, I managed to scrape better-looking embryos from inside the zooids. Some embryos got activated and started cleaving. I tried to image them in the Scanned Lightsheet SPIM (Meyers SPIM) since it has temperature control in the chamber and is adapted to small samples. Embryos were stained with a membrane marker (FM 4-64). Unfortunately, we only manage to acquire a small time-lapse from an embryo that was not healthy:

-- BRYO TIMELAPSE --

Fixed samples
I brought different stages of bryozoan embryos and larvae. There are two combinations of staining, Sytox Green + Phalloidin Alexa 647 and DAPI + Bodipy FL.