Beads

This page is about fluorescent beads, used as fiduciary markers for multi-view registration in light sheet microscopy.

Estapor Fluorescent Microspheres

 * website
 * stock concentration for SPIM: 14 µl 0.5 µm beads in 14 ml medium (sample dependent, e.g. PBS/T for fruit fly, E3 for zebrafish)
 * extended vortexing and/or treatment in ultrasonic bath is crucial for homogeneous bead distribution and successful bead detection

F-XC050

 * diameter 0.5 µm
 * "green" emission (see spectra)
 * EX 488 nm
 * appliations:
 * fiduciary marker in "green" detection channel for bright "green" fluorescence
 * evaluation/adjustment of red-green overlay

F-Y050

 * diameter 0.5 µm
 * "yellow" emission (see spectra)
 * EX 488/561 nm
 * applications:
 * fiduciary marker in "green" detection channel for weak "green" fluorescence
 * fiduciary marker in "red" detection channel for "green" fluorescence