File:Bryomov.ogv

First bryozoan embryo to be imaged live with SPIM. Stained with FM 4-64 membrane staining and imaged on a Scanned Lightsheet SPIM (Myers SPIM at MPI-CBG Dresden). Embryo was not-healthy and was used to optimize the image and test the time-lapse.

201 planes were acquired every 10s for 48 timepoints and excited with 515 nm laser. Z-stacks were focused with Fiji > Time Lapse > Gaussian-based stack focuser.