Team SPIMApes

Building our openSPIM
T configuration dual excitation (488, 561) microscope



=Yong's Projects at the 2014 EMBO LSM Practical Course=

Project A: Image Zebrafish Brain via Ptf1a-GFP
Pancreas transcription factor 1 subunit alpha (Ptf1a) is a protein that in humans is encoded by the PTF1A gene. This gene encodes a protein that is a component of the pancreas transcription factor 1 complex (PTF1) and is known to have a role in mammalian pancreatic development. Ptf1a is also shown to play an important role in the neurogenesis of different central nervous system structures. In particular, Ptf1a is important for the generation of many inhibitory (primarily γ-aminobutyric acid (GABA)-ergic) interneurons in different areas, such as the spinal cord and cerebellum. In this project, we imaged a transgenic zebrafish line expressing fluorescent Ptf1a-GFP fused proteins, with an emphasize on the structures in the brain.

The major locations of cells expressing Ptf1a-GFP are at the regions of hindbrain, retina, and pancreas.

Project B: Image Zebrafish Brain via HuC-GFP
=Marketa's Project= Primordium development in hypocotyl explants. Microscope: mDLSM. Objective: 40x