Fep tube cleaning

material

 * FEP tubes (Bola S1815-04, inner diameter 0.8 mm, outer diameter 1.6 mm)
 * ultrasonicator
 * 1 M NaOH (Merck)
 * 0.5 M NaOH
 * double-distilled H2O
 * 70% ethanol
 * syringe filter (Millex-HV PVDF 0.45 µm)
 * B. Braun Omnifix F Solo 1 ml Syringe
 * B. Braun needle (100 Sterican, blunt)

procedure

 * 1) flush tubes with 1 M NaOH, use syringe with attached needle
 * 2) transfer flushed tubes to fresh Falcon with 0.5 M NaOH, use forceps
 * 3) put the Falcon in an ultrasonic bath for 10 min
 * 4) from now on, touch the tubes only with gloves and/or forceps!
 * 5) transfer the tubes from the Falcon into small basin with ddH2O and flush them with ddH2O
 * 6) flush the tubes with 70% EtOH
 * 7) transfer the tubes to a fresh Falcon with 70% EtOH
 * 8) put the Falcon in an ultrasonic bath for 10 min
 * 9) transfer the tubes to a fresh Falcon with ddH2O for storage