Registration

= Bead Based Registration =

The first step in the SPIMage processing pipeline, after re-saving the ome.tiffs as .tif, is to register the views within each timepoint. We will use for that the bead based registration plug-in in Fiji. The principles of the plug-in are described on the Fiji wiki page SPIM registration method while the parameters are discussed in SPIM bead registration. Here we present a step by step recipe for performing the bead based registration on OpenSPIM data.